皖南医学院学报

目的:研究抗炎消肿冻疮膏(AIDO)的防治效果及机制。方法:将雄性SD大鼠随机分为Control、Model、Positive、冻疮膏低(LAIDO)和冻疮膏高(HAIDO)组，每组8只。药物组连续7 d大鼠右后足涂抹冻疮膏，Positive组0.15 g冻疮膏，LAIDO、HAIDO组分别用0.05、0.15 g AIDO。第7天用药1 h后，除Control组外，其余大鼠右后足踝关节以下部分浸泡于冷冻液(6份碎冰+1份氯化钠)中5 min构造冻疮模型。测大鼠右后足足跖厚度，计算肿胀率;计算脏器指数;检测血清中NO、TNF-α、IL-1β、ICAM-1、IL-10、T-SOD、GSH、CAT、MDA、CORT、CRH和ACTH含量及冻伤足中PGE₂含量;采用HE染色法观察冻伤足跖组织病理学变化。结果:AIDO使冻疮造模后的大鼠各个时间段的肿胀率均下降(P<0.01)。AIDO对胸腺、肾上腺和脾指数的影响和Model组相比，差异均无统计学意义(P>0.05)。AIDO使冻疮造模后的大鼠体内NO、MDA、ICAM-1、IL-1β、TNF-α、PGE₂、ACTH、CRH和CORT含量均降低(P<0.05),CAT、T-SOD、GSH和IL-10活性均上升(P<0.05)。AIDO使冻疮造模后的大鼠足组织中炎性细胞均有不同程度减少，水肿均有不同程度减轻。结论:该冻疮膏有良好的抗炎消肿作用，其抗炎消肿作用与抑制炎症因子释放和氧化应激及下丘脑-垂体-肾上腺皮质功能轴(HPA)相关。

Objective: To investigate the preventive and therapeutic effects and mechanism of the anti-inflammatory and detumescent frostbite ointment(AIDO).Methods: Male SD rats were randomly divided into control,model,positive,low-dose AIDO(LAIDO),and high-dose AIDO(HAIDO) groups,with 8 rats in each group.Chilblain ointment was topically applied to the right hind paw of the rats in the medicated groups for 7 consecutive days in a daily dose of 0. 15 g for the rats in positive group,and 0. 05 g and 0. 15 g for those in the LAIDO and HAIDO groups,respectively.On the 7^thday,one hour after medication,all the rats except those in the control group were subjected to a frostbite model by immersing the right hind paw below the ankle joint in a freezing mixture(six aliquots of crushed ice to one part sodium chloride) for 5 minutes.The following parameters were measured: the plantar thickness of the right hind paw(for calculating the swelling rate); the organ indices; serum levels of NO,TNF-α,IL-1β,ICAM-1,IL-10,T-SOD,GSH,CAT,MDA,CORT,CRH,and ACTH; and the PGE₂ content in the frostbitten foot.Hematoxylin-eosin(HE) staining was used to observe the pathological changes in the plantar tissues of the frostbitten paw.Results: AIDO significantly reduced the swelling rate at all time points after frostbite modeling in rats(P<0. 01).Compared with the model group,AIDO showed no significant effects on thymus,adrenal gland,and spleen indices(P> 0. 05). AIDO caused decrease of NO,MDA,ICAM-1,IL-1β,TNF-α,PGE₂,ACTH,CRH,and CORT levels(P<0. 05),while boosted the activities of CAT,T-SOD,GSH,and IL-10 in rats after frostbite modeling(P<0. 05).AIDO also led to varying degrees of reduction in the inflammatory cells and alleviation of edema in the foot tissues of rats with frostbite modeling. Conclusion: AIDO exhibits good anti-inflammatory and detumescent effects,which are associated with the inhibition of inflammatory factor release,reduction of oxidative stress,and modulation of the hypothalamic-pituitary-adrenal(HPA) axis.